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Additionally, the controlled release of gliotransmitters (e.g., glutamate) from astrocytic hemichannelsmembrane channels between cells and the extracellular space made up of connexin proteinsdirectly to synapses modulates synaptic transmission and plasticity, and was even shown to be essential for behavioral outputs including fear memory consolidation in rats (~2-months-old

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The pre-stained biofilm was then allowed to dry for 15 min at 37C, after which it was dissolved using 80% v/v ethanol and transferred into new 96-well plate for biomass quantification at OD 550 nm using a Tecan plate reader (Infinite M1000 pro)